Acute exercise-induced glycocalyx shedding does not differ between exercise modalities, but is associated with total antioxidative capacity

OBJECTIVES Regular physical exercise is known to protect endothelial integrity. It has been proposed that acute exercise-induced changes of the (anti-)oxidative system influence early (glycocalyx shedding) and sustained activation (shedding cells, ECs) as well endothelial-cell repair by circulating hematopoietic stem progenitor cells (HPCs). However, results are not conclusive data in trained participants performing different modalities lacking. DESIGN Eighteen healthy, well-trained (9 runners, 9 cyclists; age: 29.7 ± 4.2 yrs) performed a strenuous session consisting 4 bouts 4-min high-intensity with decreasing power profile 3-min low-intensity in-between. METHODS Average power/speed intense phases was 85% peak achieved previous incremental test. Before shortly after exercise, total oxidative antioxidative capacities (TAC), shedding syndecan-1, heparan sulfate, hyaluronan, ECs, HPCs were investigated. RESULTS TAC decreased from 1.81 ± 0.42 nmol/L 1.47 ± 0.23 nmol/L post-exercise (p = 0.010) only runners. Exercise-induced enhanced post-exercise- syndecan-1: 103.2 ± 63.3 ng/mL 111.3 ± 71.3 ng/mL, sulfate: 2637.9 ± 800.1 ng/mL 3197.1 ± 1416.3 ng/mL, both p < 0.05; hyaluronan: 84.3 ± 21.8 ng/mL 121.4 ± 29.4 ng/mL, ECs: 6.6 ± 4.5 cells/μL 9.5 ± 6.2 cells/μL, p < 0.01; between negatively related concentrations post-exercise. HPC proportions self-renewal ability negatively, while EC positively associated hyaluronan concentrations. CONCLUSIONS These highlight importance prevent endothelium vascular injury - independent modality participants. Endothelial-cell hyluronan signaling, possibly similar mechanism wound repair.